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high-density peptide array  (PEPperPRINT gmbh)

 
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    PEPperPRINT gmbh high-density peptide array
    High Density Peptide Array, supplied by PEPperPRINT gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high-density+peptide+arrays/custom+designed+commercial+high+density+peptide+chip++hdpchip+/pmc11491155-141-25-20
    Average 90 stars, based on 1 article reviews
    high-density peptide array - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Impact of Antibodies and Strain Polymorphisms on Cytomegalovirus Entry and Spread in Fibroblasts and Epithelial Cells
    Article Snippet: High-density peptide arrays were custom-printed at PEPperPRINT (Cambridge, MA).

    Peptide Microarray:

    Article Title: Impact of Antibodies and Strain Polymorphisms on Cytomegalovirus Entry and Spread in Fibroblasts and Epithelial Cells
    Article Snippet: Membranes 146 on A pril 8, 2017 by U N IV E R S IT Y O F A R IZ O N A LIB R A R Y http://jvi.asm .org/ D ow nloaded from Cui et al. Impact of antibodies on CMV entry and spread 9 were again washed with PBS with 0.2% Tween-20 and bound horse radish peroxidase-147 conjugated antibody was detected using SuperSignal west Pico chemiluminescent substrate 148 (Pierce) and exposure of X-ray film. .. 149 150 Epitope mapping by peptide array and ELISA 151 High-density peptide arrays were custom-printed at PEPperPRINT 152 (www.pepperprint.com). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Impact of Antibodies and Strain Polymorphisms on Cytomegalovirus Entry and Spread in Fibroblasts and Epithelial Cells
    Article Snippet: Membranes 146 on A pril 8, 2017 by U N IV E R S IT Y O F A R IZ O N A LIB R A R Y http://jvi.asm .org/ D ow nloaded from Cui et al. Impact of antibodies on CMV entry and spread 9 were again washed with PBS with 0.2% Tween-20 and bound horse radish peroxidase-147 conjugated antibody was detected using SuperSignal west Pico chemiluminescent substrate 148 (Pierce) and exposure of X-ray film. .. 149 150 Epitope mapping by peptide array and ELISA 151 High-density peptide arrays were custom-printed at PEPperPRINT 152 (www.pepperprint.com). ..



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    Image Search Results


    High-density peptide arrays (HDPA) provide high-resolution antibody epitope maps across the SARS-CoV-2 proteome (A) Overview of analytical pipeline. The proteome of SARS-CoV-2 was translated into 15-mer overlapping peptides with a peptide-to-peptide overlap of 13 amino acids. The resulting individual peptides were printed in duplicates on the microarray. Sera from confirmed SARS-CoV-2-positive and -negative individuals were incubated on PEPperCHIP the HDPA. Serum antibody binding was visualized using respective fluorescently labeled secondary antibodies (anti-human IgG and anti-human IgA). Image acquisition and data quantification resulted in epitope-specific antibody profiles for SARS-CoV-2. (B) Average relative fluorescent units (RFU) profiles and peptide coverages are plotted across the SARS-CoV-2 proteome (ORF1A, ORF1B, Spike (S) protein, Envelope (E) protein, Membrane (M) glycoprotein, Nucleocapsid (N) phosphoprotein). Antibody responses to each linear 15-mer peptide were mapped across the SARS-CoV-2 proteome and average RFU calculated for each amino acid residue. The normalized positional ‘epitope coverage’ at each protein residue location is defined as the ratio of total peptides mapped to each position by the total expected peptides (see section). ‘Hotspots’ can be seen as spiked in the RFU or coverage distributions. (C) Comparison of mean RFU (log-scale) between SARS-CoV-2-positive and -negative sample groups for each viral protein. (unpaired t-test, ns: p > 0.05; ∗: p ≤ 0.05; ∗∗: p ≤ 0.01; ∗∗∗: p ≤ 0.001; ∗∗∗∗: p ≤ 0.0001).

    Journal: iScience

    Article Title: Selection for immune evasion in SARS-CoV-2 revealed by high-resolution epitope mapping and sequence analysis

    doi: 10.1016/j.isci.2023.107394

    Figure Lengend Snippet: High-density peptide arrays (HDPA) provide high-resolution antibody epitope maps across the SARS-CoV-2 proteome (A) Overview of analytical pipeline. The proteome of SARS-CoV-2 was translated into 15-mer overlapping peptides with a peptide-to-peptide overlap of 13 amino acids. The resulting individual peptides were printed in duplicates on the microarray. Sera from confirmed SARS-CoV-2-positive and -negative individuals were incubated on PEPperCHIP the HDPA. Serum antibody binding was visualized using respective fluorescently labeled secondary antibodies (anti-human IgG and anti-human IgA). Image acquisition and data quantification resulted in epitope-specific antibody profiles for SARS-CoV-2. (B) Average relative fluorescent units (RFU) profiles and peptide coverages are plotted across the SARS-CoV-2 proteome (ORF1A, ORF1B, Spike (S) protein, Envelope (E) protein, Membrane (M) glycoprotein, Nucleocapsid (N) phosphoprotein). Antibody responses to each linear 15-mer peptide were mapped across the SARS-CoV-2 proteome and average RFU calculated for each amino acid residue. The normalized positional ‘epitope coverage’ at each protein residue location is defined as the ratio of total peptides mapped to each position by the total expected peptides (see section). ‘Hotspots’ can be seen as spiked in the RFU or coverage distributions. (C) Comparison of mean RFU (log-scale) between SARS-CoV-2-positive and -negative sample groups for each viral protein. (unpaired t-test, ns: p > 0.05; ∗: p ≤ 0.05; ∗∗: p ≤ 0.01; ∗∗∗: p ≤ 0.001; ∗∗∗∗: p ≤ 0.0001).

    Article Snippet: To analyze the antibody responses to SARS-CoV-2 at the epitope level we used a recently developed high-density peptide array (HDPA), the PEPperCHIP Microarray (PEPperPRINT GmbH, Germany), covering the proteome of the SARS-CoV-2 isolate Wuhan-Hu-1 as well as the four seasonal hCoVs OC43, HKU1, NL63 and 229E (see for accession numbers used).

    Techniques: Microarray, Incubation, Binding Assay, Labeling, Membrane, Residue, Comparison